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KMID : 0384119930130030445
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 3 p.445 ~ p.460
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Clinical Application of Polymerase Chain Reaction for Diagnosis of Tuberculosis
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전창호
하경임/김정숙/김경동/이영현/양창헌/안우섭
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Abstract
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To assess the sensitivity and specificity of polymerase chain reaction(PCR) for diagnosis of tuberculosis, the authors attempted PCR using insertional sequence of Eisenach as primers and detected PCR products by agarose gel electrophoresis. The
mycobacterial species used in the study were 9 species of MOTT(Mycobcateria other than tuberculosis), 2 strains of M. tuberculosis and 229 sputum specimens from the patients visiting Dongguk University Pohang and Kyungju Hospitals, and Yeungnam
University Hospital. The results obtained by PCR were compared with those tested by microbiological methods and with clinical data. The PCR products were detected by agarose gel electrophoresis using 100 fg of input DNA and also from the
specimens
which
even disclosed one colony on Loewenstein-Jensen media. Double PCR was able to detect as small amount as only l fg of input DNA. Senventy-seven(81.1%) of 95 cases of pulmonary tuberculosis caused by M. tuberculosis revealed PCR positivity, while
no
product was detected in the specimens from 99 patients of non-tuberculous pulmonary disease and other systemic disorders, resulting in 100% of specificity. The sensitivity of PCR resuits was 2.0 and 1.4 times of that of AFB stain and culture,
respectively. Eighteen (18.4%) of 98 cases of pulmonary tuberculosis with negative result by traditional microbiclogic methods exhibited PCR positivity.
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KEYWORD
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